Effects of Xingpi Yang'er granule on serum gastrin, plasma motilin, and somatostatin contents in children patients with pneumonia induced diarrhea / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the effects of Xingpi Yang'er Granule (XYG) on serum gastrin (GAS), plasma motilin (MOT), and somatostatin (SS) in children patients with pneumonia induced diarrhea.</p><p><b>METHODS</b>Recruited were 120 children inpatients with pneumonia induced diarrhea at the Department of Pediatrics, Liaocheng People's Hospital from June 2011 to June 2012. They were randomly assigned to two groups, the treatment group and the control group, 60 in each group. Those in the treatment group were treated with XYG, while those in the control group were treated with Live Combined Bifidobacterium and Lactobacillus Tablets. Besides, 30 healthy children who received physical examinations at our hospital were recruited as the healthy control group. The clinical efficacy, changes of GAS, MOT, and SS contents were observed.</p><p><b>RESULTS</b>The total effective rate was 95.0% in the treatment group and 93.3% in the control group, showing no statistical difference (P > 0.05). Compared with healthy control group, the GAS and MOT contents increased, and SS decreased before treatment in the other two groups (P < 0.05). Compared with the same group before treatment, GAS and MOT contents obviously decreased, and SS increased in the other two groups after treatment (P<0.05). Compared with the control group at the same time point, GAS and MOT decreased, and SS increased in the treatment group after treatment, showing statistical differences (P < 0.05).</p><p><b>CONCLUSIONS</b>The levels of GAS, MOT, and SS were obviously changed in children patients with pneumonia induced diarrhea. XYG had obvious regulation on their GAS, MOT and SS contents.</p>

Effect of MGF-Ct24E and mechanical stimulation on gene expression of osteoblasts / 医用生物力学

Objective To identify the differentially expressed genes of osteoblasts under the stimulation of mechano growth factor E peptide( MGF-Ct24E) and mechanical stress by microarray analysis. Methods Primary osteoblasts were cultured in vitro, which were subjected to mechanical stimulation(with the mechanical strain of 12% and frequency of 0.5 Hz) and MGF-Ct24E treatment(50 mg/L), respectively. The gene expression profiles were analysed by cDNA microarrys and quantitative PCR was used to validate the microarray data. ResultsCompared with the control group, 1 866 genes were found to have differentially expressed in the mechanical loading group, in which 1 113 genes were up-regulated, while 753 genes were down-regulated. 1 178 genes were found to have differentially expressed in the MGF-Ct24E group, in which 796 genes were up-regulated and 382 genes were down-regulated. GO analysis suggested that the gene expression profile of MGF-Ct24E group was consistent with that of the mechanical loading group and differentially expressed genes were mainly involved in cell proliferation and differentiation, response to mechanical stress and mechaotransduction. ConclusionsThe microarray analysis showed that MGF-Ct24E treatment had similar effects with the mechanical loading on the gene expression of osteoblasts, which might provide a novel approach to study the usage of MGF-Ct24E for treating bone repair in the absence of mechanical stimulation.